Molecular assays to detect the presence and viability of Phytophthora ramorum and Grosmannia clavigera

Version 1.0
Feau, Nicolas; Wong, Barbara; Hamelin, Richard, 2020, "Molecular assays to detect the presence and viability of Phytophthora ramorum and Grosmannia clavigera", https://doi.org/10.5683/SP2/ZSAWXJ, Borealis, V1
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Description
Abstract

Protocols for the RNA and DNA extractions, cDNA synthesis and qPCR and RT-qPCR for detecting and assessing viability of the Phytophthora ramorum and Grosmannia clavigera tree pathogens.

(2020-01-07)
Methods

Fifty to 80 mg of wood scrapings were collected at each inoculation point from eight logs of four conifer species and used for the simultaneous extractions of gDNA and mRNA.

Using the diluted RNA, cDNA, synthesis was performed using the QuantiTect Reverse Transcription Kit (QIAGEN).

(2020-01-07)
Subject Other
Keyword RNA and DNA extraction, cDNA synthesis, qPCR, RT-qPCR, Phytophthora ramorum, Grosmannia clavigera, tree pathogen, QuantiTect Reverse Transcription Kit (QIAGEN)
Notes

Dryad version number: 2

Version status: submitted

Dryad curation status: Published

Sharing link: https://datadryad.org/stash/share/0aNNOOZ1MhDyvvih6bARA7n35F7br_OYXXy-nj8iXHQ

Storage size: 40927

Visibility: public

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dryad_jq2bvq861.json
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Published May 20, 2021
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Molecular_assays_to_detect_the_presence_and_viability_of_Phytophthora_ramorum_and_Grosmannia_clavigera_(1).docx
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Published May 20, 2021
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MD5: d90b5d009ee3f265e94ade6340965eb5
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