Live Cell Imaging of Vaccinia Virus Recombination
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1 to 6 of 6 Results
Video 6
Sep 12, 2016
Paszkowski, Patrick, 2016, "Video 6", https://doi.org/10.7939/DVN/10875, Borealis, V1
GFPcro BSC-40 cells were transfected with linearized pmCherry-cro plasmid 4 hours prior to infecting with VACV-pE/L-mCherry(t) at MOI = 5. Images were collected immediately after initiating the infection and the appearance of new GFPcro labeled DNA was used to track factory devel...
Video 4
Sep 12, 2016
Paszkowski, Patrick, 2016, "Video 4", https://doi.org/10.7939/DVN/10873, Borealis, V1
mCherry-cro BSC-40 cells were infected at a MOI = 5 with a virus encoding YFP-tagged to the A5 protein (VACV-A5-YFP), and tracked using live cell microscopy to detect the appearance of late stage A5-YFP protein.
Video 2
Sep 12, 2016
Paszkowski, Patrick, 2016, "Video 2", https://doi.org/10.7939/DVN/10871, Borealis, V1
GFPcro BSC-40 cells were co-infected at a total MOI = 5 with VACV-pE/L-mCherry(t) and VACV-mCherry-cro viruses, and tracked using live cell microscopy to detect the appearance of recombinant mCherry-cro protein.
Video 1
Sep 12, 2016
Paszkowski, Patrick, 2016, "Video 1", https://doi.org/10.7939/DVN/10868, Borealis, V1
Live Cell Imaging - GFPcro BSC-40 cells were infected with VACV-pE/L-mCherry-cro at a MOI = 5, and the red and green fluorescence then tracked over time, collecting images 5 minutes apart, across 10 different fields of view (only a single representative field is shown).
Video 5
Sep 12, 2016
Paszkowski, Patrick, 2016, "Video 5", https://doi.org/10.7939/DVN/10874, Borealis, V1
GFPcro BSC-40 cells were infected with VACV-pE/L-mCherry(dup) at a MOI = 0.5 to favor infections with a single particle. Two different cells are tracked here from the time of factory development: one infected by an actively recombining virus, and another presumed to be infected w...
Video 3
Sep 12, 2016
Paszkowski, Patrick, 2016, "Video 3", https://doi.org/10.7939/DVN/10872, Borealis, V1
GFPcro BSC-40 cells were infected at a MOI = 5 with a virus encoding mCherry-tagged to the I1 protein (VACV-I1L-mCherry), and tracked using live cell microscopy to detect the appearance of intermediate stage mCherry protein.
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